Rational Design of Monodisperse Mesoporous Silica Nanoparticles for Phytase Immobilization

Monodisperse mesoporous silica nanoparticles (MMSNs) with fractal structures were synthesized via a facile, one-pot, surfactant-free process under the well-known Stüber synthesis condition. It was characterized by scanning electron microscope, transmission electron microscopy, and N₂ adsorption-desorption isotherms. Phytase was immobilized on the MMSNs by physical adsorption. The enzyme loading capacity, activity, and release profile were measured by a faster and more reliable assay method, which was based on the hydrolysis of para-nitrophenylphosphate. The results show that the fractal structures have an important influence on the phytase capacity, and the releasing results also illustrated that phytase immobilized on MMSNs possessed the smallest releasing amounts under acidic conditions (pH = 3).