Altererythrobacter segetis sp. nov., Isolated from Farmland Soil

A Gram-stain-negative bacterium, designated YJ20^T, was isolated from rhizosphere soil of a spinach farmland at Shinan in Korea. Strain YJ20^T was found to be aerobic, non-motile rods which can grow at 10-33 °C (optimum, 28 °C), at pH 6.5-8.5 (optimum, pH 6.5-7.5) and in the absence of NaCl. The 16S rRNA gene sequence analysis showed that strain YJ20^T belongs to the genus Altererythrobacter with moderate sequence similarities to Altererythrobacter dongtanensis KCTC 22672^T (96.8%), Altererythrobacter soli MN-1^T (96.6%) and Altererythrobacter xinjiangensis S3-63^T (96.5%). The phylogenomic analysis based on the whole-genome sequence demonstrated that strain YJ20^T formed a distinct phyletic line with Altererythrobacter soli MN-1^T and Altererythrobacter salegens XY-R17^T showing average nucleotide identity (ANI) values of 79.4 and 77.5%, respectively. The predominant ubiquinone was identified as Q-10, and the major fatty acids were C_17:1 ω6c, C_18:1 ω7c and C_15:0 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidycholin, sphingoglycolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the genome was determined to be 66.3 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic and phylogenomic analyses in this study, strain YJ20^T is considered to represent a novel species in the genus Altererythrobacter, for which the name Altererythrobacter segetis sp. nov. is proposed. The type strain is YJ20^T (= KACC 19554^T = NBRC 113199^T).