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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Trypsin Detection Strategies: A Review.

Jasvir Kaur1, Prabhat K Singh1

  • 1Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.

Critical Reviews in Analytical Chemistry
|November 30, 2020
PubMed
Summary

Sensitive biosensors are crucial for detecting trypsin, a pancreatic enzyme vital in various applications. This review overviews optical, electrochemical, and other systems for trypsin detection, highlighting a highly sensitive photo-electrochemical immunosensor.

Keywords:
Aggregationfluorescencegold nanoparticlesprotaminesupramolecular complextrypsin

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Area of Science:

  • Biochemistry and Biosensor Technology
  • Enzyme Analysis and Diagnostics

Background:

  • Trypsin, a pancreatic serine protease, is essential in food technology, proteomics, and as a disease biomarker.
  • The need for simple, sensitive, and selective biosensors for trypsin detection is significant.

Purpose of the Study:

  • To provide a comprehensive overview of biosensing systems developed for trypsin detection over the past decade.
  • To categorize and critically analyze various sensor types based on their signal output and components.

Main Methods:

  • Categorization of biosensors into optical (fluorescence, colorimetric, SPR, liquid crystals), electrochemical (photo-electrochemical, nanopore), piezoelectric, and ELISA-based systems.
  • Analysis of biosensor construction using natural proteins, nucleic acids, nanoparticles, dyes, and polymers as interacting units.
  • Critical evaluation of working principles, detection limits, linearity, and response times.

Main Results:

  • A wide array of biosensing strategies for trypsin detection has been developed.
  • Photo-electrochemical anti-trypsin immunosensors demonstrate exceptional sensitivity.
  • The most sensitive sensor achieved a limit of detection of 0.02 ng/mL and a linearity range of 0.10-100 ng/mL.

Conclusions:

  • Biosensors offer promising solutions for accurate and efficient trypsin quantification.
  • Photo-electrochemical immunosensors represent a leading technology for highly sensitive trypsin detection.