Glycomyces terrestris sp. nov., isolated from extremely arid soil from Yuanmou Earth Forest

A Gram-stain-positive, mycelium-forming actinobacterium, YIM 121974^T was isolated from an extreme arid soil sample collected at Yuanmou Earth Forest, Yunnan Province, PR China. Classification using a polyphasic approach suggested that strain YIM 121974^T belonged to the genus Glycomyces and was closely related to Glycomyces dulcitolivorans SJ-25^T (98.3 %), Glycomyces scopariae YIM 56256^T (98 %), Glycomyces mayteni YIM 61331^T (97.9 %), Glycomyces albidus NEAU-7082^T (97.9 %), Glycomyces sambucus CGMCC 4.3147^T (97.7 %), Glycomyces artemisiae IXS4^T (97.6 %) and Glycomyces parisis CPCC 204357^T (97.5 %), but could be distinguished from its closest relatives by a combination of phenotypic and phylogenetic features. Average nucleotide identity values of YIM 121974^T to its closest phylogenetic neighbours were 70.7-88.9 %, which are lower than the threshold of 95 %. The digital DNA-DNA hybridization values between YIM 121974^T and these relative species were 18.0-36.3 %, which are also well below the cut-off value (>70 %) for species delineation. The DNA G+C content of strain YIM 121974^T was 72.3 mol% (draft genome sequence). The predominant menaquinone was MK-11. The phospholipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid and glycolipid. The major fatty acid compositions were iso-C_16 : 0, anteiso-C_15 : 0 and anteiso-C_17 : 0. The draft genome of isolate YIM 121974^T was found to contain 11 secondary metabolite biosynthesis gene clusters by using the antiSMASH server. Based on the above observations, strain YIM 121974^T could be distinguished from closely related species belonging to the genus Glycomyces. Thus, strain YIM 121974^T represents a novel species of the genus Glycomyces, for which the name Glycomyces terrestris sp. nov. is proposed. The type strain is YIM 121974^T (=KCTC 39870^T=DSM 106742^T).