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Silicon-assisted surface enhanced fluorescence toward improved assay performances
Ruohu Zhang1, Hui Lu1, Shenfei Zong1
1Advanced Photonics Center, Southeast University, Nanjing 210096, Jiangsu, People's Republic of China.
Nanotechnology
|November 30, 2020
Summary
This study introduces silicon-assisted surface enhanced fluorescence (SEF) assays. This novel method enhances fluorescence signals and suppresses background noise, significantly improving detection limits for various applications.
Area of Science:
- Materials Science
- Analytical Chemistry
- Nanotechnology
Background:
- Surface-enhanced fluorescence (SEF) assays are crucial for sensitive detection.
- Achieving both low background noise and high signal enhancement in SEF remains a challenge.
Purpose of the Study:
- To develop a novel silicon-assisted SEF scheme combining background suppression and signal enhancement.
- To investigate the mechanism behind the enhanced SEF effect.
Main Methods:
- Utilizing P-doped, (100) oriented silicon substrates to quench fluorescence.
- Depositing silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) on Rose Bengal (RB) coated silicon.
- Investigating the SEF mechanism through fluorescence spectroscopy and theoretical considerations.
Main Results:
- Silicon substrate effectively quenched Rose Bengal fluorescence, reducing background signal.
- Silver nanoparticles enhanced fluorescence by up to 290-fold.
- Gold nanoparticles also demonstrated SEF, offering improved stability and biocompatibility.
Conclusions:
- The silicon-assisted SEF scheme significantly enhances fluorescence sensitivity and lowers the limit of detection (LOD).
- The combined effects of fluorescence quenching and nanoparticle-induced enhancement are key to the improved performance.
- This approach shows great potential for developing highly sensitive SEF-based assays.
