Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thioredoxin system modulates metabolic and stomatal responses to elevated CO<sub>2</sub> in Arabidopsis.

The New phytologist·2026
Same author

Remodeling of the leaf lipidome during cold acclimation, de-acclimation and re-acclimation influences the frost tolerance of Festuca arundinacea.

Plant & cell physiology·2026
Same author

Exploring the diversity of the CO2-concentrating mechanism in different C4 subtypes.

Journal of experimental botany·2026
Same author

Genomic imprinting in an early-diverging angiosperm reveals an ancient mechanism for seed initiation in flowering plants.

The New phytologist·2025
Same author

Nitrogen Fertilizer Exerts Little Impact on Highbush Blueberry Floral Chemistry, Pollinator Visitation, Yield, and Fruit Quality.

Physiologia plantarum·2025
Same author

Characterization of the cyclic dipeptide cyclo(His-Pro) in Arabidopsis.

Plant physiology·2025

Related Experiment Video

Updated: Nov 28, 2025

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

825

Plant Single-Cell Metabolomics-Challenges and Perspectives.

Leonardo Perez de Souza1, Monica Borghi2, Alisdair Fernie1

  • 1Max Planck Institute of Molecular Plant Physiology, Am Müehlenberg 1, Golm, 14476 Potsdam, Germany.

International Journal of Molecular Sciences
|December 1, 2020
PubMed
Summary

Single-cell omics, including genomics and transcriptomics, are powerful tools. Spatially resolved metabolomics is emerging for single-cell analysis, though challenges remain in achieving full metabolome coverage.

Keywords:
cell type specific metabolismmass spectrometry imagingmetabolomicssingle-cell

More Related Videos

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.0K
Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos
12:16

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Published on: December 22, 2017

20.7K

Related Experiment Videos

Last Updated: Nov 28, 2025

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

825
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.0K
Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos
12:16

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Published on: December 22, 2017

20.7K

Area of Science:

  • Single-cell biology
  • Systems biology
  • Plant science

Background:

  • Omics approaches (genomics, transcriptomics, proteomics, metabolomics) are foundational in modern biology.
  • Single-cell genomics and transcriptomics are established tools for tissue-level analysis.
  • Single-cell proteomics is advancing, but single-cell metabolomics faces significant challenges.

Purpose of the Study:

  • To review state-of-the-art technologies for spatially resolved metabolomics.
  • To discuss challenges in achieving single-cell metabolome coverage.
  • To explore contributions to understanding plant single-cell metabolism and future directions.

Main Methods:

  • Review of current technologies for spatially resolved metabolomics.
  • Analysis of limitations and challenges in single-cell metabolomics.
  • Discussion of existing research on plant single-cell metabolism.

Main Results:

  • Significant advancements in single-cell genomics and transcriptomics.
  • Emerging technologies for spatially resolved metabolomics show promise.
  • Metabolomics lags behind other omics in single-cell resolution and coverage.

Conclusions:

  • Spatially resolved metabolomics is crucial for understanding cellular heterogeneity.
  • Overcoming technical hurdles is essential for comprehensive single-cell metabolome analysis.
  • Future research will likely focus on integrating advanced metabolomics with other omics for deeper biological insights, particularly in plant systems.