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Published on: November 18, 2016
Platelet proteome changes in dogs with congestive heart failure
Pinar Levent1, Meriç Kocaturk1, Emel Akgun2
1Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey.
Insights
Platelet protein changes were identified in dogs with congestive heart failure (CHF). This study reveals altered platelet proteomes in canine CHF, offering insights into disease mechanisms and potential therapeutic targets.
Area of Science:
- Cardiovascular Science
- Proteomics
- Canine Medicine
Background:
- Platelets are crucial in cardiovascular disease development.
- Platelet protein alterations are implicated in human heart disease pathophysiology.
- The role of platelets in canine congestive heart failure (CHF) is not well understood.
Purpose of the Study:
- To investigate global platelet proteome changes in dogs with CHF.
- To clarify the potential role of platelets in the physiopathology of canine CHF.
- To establish a baseline for understanding platelet function in canine heart disease.
Main Methods:
- Compared platelet proteomes of healthy dogs (n=10) with dogs experiencing acute CHF due to myxomatous mitral valve disease (MMVD, n=10).
- Utilized LC-MS based label-free differential proteome expression analysis.
- Identified and quantified proteins within canine platelet samples.
Main Results:
- Identified 104 distinct proteins in dog platelets.
- Observed significant up-regulation of 4 proteins and down-regulation of 6 proteins in acute CHF dogs.
- Noted changes in proteins involved in molecular functions, biological processes, and immune-inflammatory responses.
Conclusions:
- This study presents the first description of platelet protein composition changes in dogs with acute CHF secondary to MMVD.
- Findings enhance knowledge of canine platelet proteomes and their involvement in MMVD-induced CHF.
- Results provide a foundation for future research into the prevention and treatment of canine CHF.
Background:
Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in congestive heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n = 10) and dogs with acute CHF due to myxomatous mitral valve disease (MMVD, n = 10) were used. Acute CHF was defined based on the clinical (increased respiratory rate or difficulty breathing) and radiographic findings of pulmonary edema. Dogs Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris.
Results:
Totally 104 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in acute CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in acute CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.
Conclusion:
This study describes by first time the changes in the protein composition in platelets of dogs with acute CHF due to MMVD. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF caused by MMVD and could be a tool for further investigations about the prevention and treatment of this disease.
