Tumor Immunotherapy
T Cell Activation and Clonal Selection
Cell-mediated Immune Responses
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Updated: Nov 28, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
Published on: May 9, 2025
Andrew H Marple1, Challice L Bonifant2, Nirali N Shah3
1Department of Medical Oncology, Johns Hopkins Hospital, Baltimore, MD.
Chimeric antigen receptor (CAR) T-cell therapy offers high remission rates for acute lymphoblastic leukemia (ALL). However, challenges like relapse and resistance necessitate next-generation CAR T-cell engineering for durable patient outcomes.
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