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Updated: Jul 11, 2026
Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
Published on: December 13, 2013
Retinal wholemounts: a simple method for precise mapping
1Neuroembryology Laboratory, School of Anatomy, University of Queensland, Brisbane, Australia.
Abstract:
A simple and inexpensive method to analyse retinal wholemounts is described. It allows the definition of regularly spaced sites to map regional specializations of the retina under light microscopy. After attaching a piece of translucent millimetre graph paper behind the slide holding the preparation, the two grid patterns of the graph paper and of the eye-piece grid are used in combination for the definition of sites for analysis and the return to specific areas as small as 50-100 microns2. The present method is simpler and offers several advantages when compared to others based on X-Y movements of the microscope stage or on photographic montage.
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