Electricity from methane by Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b

Given the difficulties valorizing methane (CH₄) via catalytic routes, this study explores use of CH₄-oxidizing bacteria ("methanotrophs") for generating electricity directly from CH₄. A preconditioned methanotrophic biofilm on 3D nickel foam with reduced graphene oxide (rGO/Ni) was used as the anode in two-compartment microbial fuel cells (MFCs). This study demonstrates a proof of concept for turning CH₄ into electricity by two model methanotrophs including Methylosinus trichosposium OB3b and Methylococcus capsulatus (Bath). Both OB3b (205 mW.m^-2) and Bath (110 mW.m^-2) strains yielded a higher electricity from CH₄ when grown on rGO/Ni compared to graphite felt electrodes. Based on electrochemistry tests, molecular dynamics simulations, genome annotations and interaction analysis, a mechanistic understanding of reasons behind enhanced performance of methanotrophs grown on rGO/Ni are presented.