Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Aging01:26

Aging

450
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
450
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

3.0K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.0K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.1K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.1K
Mitochondria01:37

Mitochondria

18.2K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
18.2K
The Proteasome01:13

The Proteasome

1.3K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.3K
The Proteasome02:18

The Proteasome

4.0K
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of physiological and pathological condensates by molecular chaperones.

The FEBS journal·2025
Same author

PVRIG is Expressed on Stem-Like T Cells in Dendritic Cell-Rich Niches in Tumors and Its Blockade May Induce Immune Infiltration in Non-Inflamed Tumors.

Cancer immunology research·2024
Same author

Stress-induced transcriptional readthrough into neighboring genes is linked to intron retention.

iScience·2022
Same author

Differential roles for DNAJ isoforms in HTT-polyQ and FUS aggregation modulation revealed by chaperone screens.

Nature communications·2022
Same author

Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle Regeneration.

Cell reports·2020
Same author

Data-Modeling Identifies Conflicting Signaling Axes Governing Myoblast Proliferation and Differentiation Responses to Diverse Ligand Stimuli.

Cellular and molecular bioengineering·2019

Related Experiment Video

Updated: Nov 28, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

3.8K

Cellular proteostasis decline in human senescence.

Niv Sabath1, Flonia Levy-Adam1, Amal Younis1

  • 1Department of Biochemistry, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, 31096 Haifa, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|December 1, 2020
PubMed
Summary
This summary is machine-generated.

Proteostasis decline, crucial for aging in worms, is also intrinsic to human aging. Senescent human cells struggle to activate stress responses, impairing protein homeostasis and aging.

Keywords:
UPRchaperonesheat shock responseprotein homeostasissenescence

More Related Videos

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.1K
Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

17.6K

Related Experiment Videos

Last Updated: Nov 28, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

3.8K
Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.1K
Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

17.6K

Area of Science:

  • Cellular Biology
  • Aging Research
  • Molecular Biology

Background:

  • Proteostasis collapse is a hallmark of nematode aging.
  • Its occurrence in human senescence remained unclear.

Purpose of the Study:

  • To investigate proteostasis decline in human senescence.
  • To characterize molecular mechanisms underlying this decline.

Main Methods:

  • Transcriptome-wide analysis of gene expression, splicing, and translation.
  • Assessment of heat shock response and unfolded protein response (UPR) pathways.
  • Analysis of HSF1 and ATF6 localization.
  • Evaluation of proteasome function.

Main Results:

  • Senescent cells exhibit impaired transcriptional activation of heat shock response.
  • HSF1 nuclear localization and distribution are diminished in senescence.
  • UPR shows a decoupling, with enhanced translation but impaired transcriptional response.
  • ATF6 nuclear localization is reduced, and proteasome function is impaired in stressed senescent cells.

Conclusions:

  • Human senescence involves intrinsic proteostasis decline.
  • Deterioration in mounting dynamic stress transcriptional programs impacts proteostasis.
  • This decline is linked to human aging and impaired cellular stress responses.