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Effect of D614G Spike Variant on Immunoglobulin G, M, or A Spike Seroassay Performance
Carleen Klumpp-Thomas1,2, Heather Kalish3, Jennifer Hicks3
1National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA.
Abstract:
Emergence of a new spike protein variant (D614G) with increased infectivity has prompted many to analyze its role in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There is concern regarding whether an individual exposed to one variant of a virus will have cross-reactive memory to the second variant. Accordingly, we analyzed the serologic reactivity of both variants, and we found that antibodies from 88 donors from a high-incidence population reacted toward both the original spike and the D614 spike variant. These data suggest that patients who are exposed to either variant have cross-responsive humoral immunity. This represents an important finding both for SARS-CoV-2 disease biology and for therapeutics.
Insights
Antibodies from individuals exposed to either the original or D614G spike protein variant of SARS-CoV-2 show cross-reactive immunity. This finding is crucial for understanding severe acute respiratory syndrome coronavirus 2 disease and developing effective therapeutics.
Area of Science:
- Virology
- Immunology
- Epidemiology
Background:
- A new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein variant, D614G, has emerged with increased infectivity.
- Concerns exist about cross-reactive immune memory between different SARS-CoV-2 variants.
Purpose of the Study:
- To investigate the serologic reactivity of antibodies against both the original SARS-CoV-2 spike protein and the D614G variant.
- To determine if exposure to one variant confers cross-immune responses to another.
Main Methods:
- Analysis of serologic reactivity using samples from 88 donors from a high-incidence population.
- Testing antibody responses against both the original and D614G spike protein variants.
Main Results:
- Antibodies from all 88 donors reacted to both the original spike protein and the D614G spike variant.
- Demonstrated cross-responsive humoral immunity in patients exposed to either variant.
Conclusions:
- Exposure to either the original or D614G SARS-CoV-2 spike protein variant elicits cross-reactive humoral immunity.
- These findings have significant implications for SARS-CoV-2 disease biology and therapeutic strategies.
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