Effects of corn steep liquor on β-poly(L-malic acid) production in Aureobasidium melanogenum
Genan Wang1,2, Bingyi Shi1,2, Pan Zhang1,2
1Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin, 300457, People's Republic of China.
Abstract:
β-poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.
Insights
Corn steep liquor (CSL) enhances β-poly(L-malic acid) (PMLA) production in Aureobasidium melanogenum. This study reveals CSL boosts PMLA yield and identifies key metabolic changes, offering an efficient biotechnological approach.
Area of Science:
- Biotechnology
- Biochemistry
- Microbiology
Background:
- β-poly(L-malic acid) (PMLA) is a valuable water-soluble biopolymer with diverse applications.
- Limited microbial biosynthesis restricts PMLA's biotechnological potential.
- Corn steep liquor (CSL) is a low-cost, nutrient-rich byproduct suitable for microbial fermentation.
Purpose of the Study:
- To investigate the effect of corn steep liquor (CSL) on PMLA production by Aureobasidium melanogenum.
- To analyze the metabolomic changes in A. melanogenum in response to CSL addition.
- To identify strategies for enhancing PMLA biosynthesis.
Main Methods:
- Cultivation of Aureobasidium melanogenum with varying concentrations of CSL.
- Measurement of PMLA production, cell growth, and yield (Yp/x).
- Metabolomic analysis of intracellular metabolites using techniques like mass spectrometry.
Main Results:
- Supplementation with 3 g/L CSL significantly increased PMLA production by 32.76%.
- CSL addition led to a 41.82% increase in cell growth and a 47.43% enhancement in yield (Yp/x).
- CSL promoted the accumulation of intracellular metabolites, including amino acids, organic acids, and TCA cycle intermediates, with tyrosine identified as a key factor in PMLA biosynthesis.
Conclusions:
- Corn steep liquor (CSL) is an effective and economical additive for enhancing PMLA biosynthesis in Aureobasidium melanogenum.
- CSL addition modulates microbial metabolism, leading to improved PMLA yields.
- This study provides a viable strategy for increasing PMLA production for industrial applications.
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