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[Effect of antibiotics on IL-1 in vitro production by human monocytes]
Y Roche1, J J Pocidalo, M A Gougerot-Pocidalo
1Laboratoire d'Immunologie et d'Hématologie, CHU X. Bichat, Paris.
Abstract:
The effects of penicillin, macrolides (spiramycin and erythromycin), cephalosporins (cefaclor and cefadroxil), cycline (doxycycline) and quinolones (pefloxacin, ciprofloxacin and ofloxacin) on extracellular and cell-associated interleukin-1 activity from human monocytes were investigated in vitro. When cells were treated with 10 micrograms/ml of quinolones, cephalosporins or penicillin, no effect on IL-1 production could be detected. Using 100 micrograms/ml, extracellular IL-1 activity was found to be decreased by quinolones (about 35% of the control without antibiotic) without modification of the cell-associated IL-1 activity. Extra and intracellular IL-1 was only slightly decreased by cephalosporins, while penicillin did not alter the IL-1 activities. Spiramycin and doxycycline using 100 micrograms/ml increased extracellular IL-1 while cell-associated was decreased. A toxic effect may have been exerted by these antimicrobial agents.
Insights
Certain antibiotics, like quinolones, can decrease interleukin-1 (IL-1) activity in human monocytes. Other antibiotics, such as spiramycin and doxycycline, may increase IL-1 activity, potentially due to toxicity.
Area of Science:
- Immunology
- Pharmacology
Context:
- Human monocytes are crucial immune cells involved in inflammatory responses.
- Interleukin-1 (IL-1) is a key cytokine regulating immune and inflammatory processes.
Purpose:
- To investigate the in vitro effects of various antibiotic classes on IL-1 activity in human monocytes.
- To determine how penicillin, macrolides, cephalosporins, doxycycline, and quinolones influence extracellular and cell-associated IL-1.
Summary:
- At 100 µg/ml, quinolones significantly reduced extracellular IL-1 activity (by ~35%) without affecting cell-associated IL-1.
- Cephalosporins showed a slight decrease in both extra- and intracellular IL-1, while penicillin had no effect.
- Spiramycin and doxycycline increased extracellular IL-1 and decreased cell-associated IL-1, suggesting potential toxicity.
Impact:
- Antibiotic choice may influence immune responses by modulating IL-1 production.
- Findings highlight differential effects of antibiotics on monocyte-derived IL-1.
- Further research is needed to confirm the toxic effects and clinical implications.