Regulation of volatile fatty acid accumulation from waste: Effect of inoculum pretreatment

The study investigates the implications of waste feedstock, inoculum origin, and pretreatment on volatile fatty acids accumulation (VFA). The acidogenic fermentation of the feedstocks, rice mill effluent (RME), and brewery effluent (BE) was studied using untreated and pretreated (cyclic heat-acid shock) brewery anaerobic sludge as inoculum. The pretreatment was successful in refining and stabilizing VFA production from the feedstocks. The fermentation of RME with pretreated sludge had an enhanced acetate yield of 0.37 ± 0.02 mgCOD/mgCOD, even to odd ratio of 20.97 ± 0.08 mg/mg and the highest butyrate yield of 0.39 ± 0.01 mgCOD/mgCOD compared to untreated system. The pretreated system had stability in COD and pH profile, while VFA content depends on the origin of inoculum. Pretreatment inhibited the carbon sinks and augmented acetate-butyrate type metabolism with stable performance. The fermentation of RME by pretreated sludge produced a higher even-numbered VFAs and enhanced even to odd ratio in comparison with fermentation of BE, thereby affecting polymer composition and property. PRACTITIONER POINTS: The pretreated system had stable acidification, chemical oxygen demand, and pH profile. The pretreated system had higher acetate and butyrate yield compared to the untreated system. Rice mill effluent acidified with pretreated sludge had the highest even to odd ratio, 20.97 mg/mg. The even to odd ratio for acidification of brewery effluent was insignificant. Pretreatment, the origin of sludge, and the effluent had a regulatory effect on acidification.