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Pancreatobiliary Diversion in the Mouse.

Xijuan Chen1, Shuai Qiu1, Qiuju Li1

  • 1Graduate School, Tianjin Medical University, Tianjin, China.

European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes
|December 1, 2020
PubMed
Summary

Researchers developed a new mouse model for studying cholecystokinin (CCK) by modifying pancreatobiliary diversion (PBD). This model successfully demonstrated increased pancreatic growth due to elevated CCK levels, offering a valuable tool for CCK-related research.

Keywords:
CholecystokininMousePancreasPancreatobiliary diversion

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Area of Science:

  • Gastroenterology and Molecular Biology
  • Endocrinology
  • Surgical Research

Background:

  • Cholecystokinin (CCK) is a gut hormone regulating digestion, appetite, and pancreatic growth.
  • Its role in pancreatic carcinogenesis is significant, but molecular mechanisms remain unclear.
  • Existing animal models, like pancreatobiliary diversion (PBD), are not established in mice.

Purpose of the Study:

  • To establish a functional pancreatobiliary diversion (PBD) surgical model in mice.
  • To investigate the effects of PBD-induced hypercholecystokininemia on pancreatic growth.
  • To provide a novel tool for CCK-related research, including pancreatic cancer studies.

Main Methods:

  • Modified PBD procedures were performed on mice, with a sham operation (SO) group as control.
  • Plasma CCK levels were measured using radioimmunoassay.
  • Pancreatic weight, protein, DNA content, and cell proliferation (PCNA, BrdU) were assessed.

Main Results:

  • PBD mice exhibited significantly higher plasma CCK levels compared to SO controls (p < 0.001).
  • PBD mice showed increased pancreatic weight (p < 0.001), protein (p < 0.001), DNA (p < 0.01), and cell proliferation (p < 0.01).
  • The PBD model in mice was validated as successful in inducing hypercholecystokininemia and promoting pancreatic growth.

Conclusions:

  • The established PBD mouse model effectively mimics PBD-induced hypercholecystokininemia.
  • This model demonstrates a direct link between elevated CCK and increased pancreatic growth.
  • The PBD mouse model is a valuable resource for future research on CCK's role in pancreatic physiology and pathology, including cancer.