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Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

31.8K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

3.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.1K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Updated: Nov 27, 2025

Denaturing Urea Polyacrylamide Gel Electrophoresis Urea PAGE
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Denaturing Urea Polyacrylamide Gel Electrophoresis Urea PAGE

Published on: October 29, 2009

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Polyacrylamide Gel Electrophoresis.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |December 2, 2020
    PubMed
    Summary

    Polyacrylamide gels offer superior DNA separation and purity compared to agarose, enabling precise analysis of DNA fragments. These gels provide high resolution and capacity, ideal for demanding molecular biology applications.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Agarose gels are commonly used for DNA fragment separation.
    • Polyacrylamide gels offer potential advantages in resolution and purity.

    Purpose of the Study:

    • To detail methods for preparing and running nondenaturing polyacrylamide gels.
    • To highlight the advantages of polyacrylamide gels for DNA analysis.

    Main Methods:

    • Preparation of cross-linked polyacrylamide gels.
    • Running nondenaturing polyacrylamide gels for DNA separation.
    • Detection of DNA in polyacrylamide gels via staining.

    Main Results:

    • Polyacrylamide gels provide high-resolution separation of DNA fragments (0.1% size difference).

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  • These gels accommodate larger DNA quantities (up to 10 µg) per slot without resolution loss.
  • DNA recovered from polyacrylamide gels is highly pure, suitable for sensitive applications like microinjection.

    • Conclusions:

    • Polyacrylamide gels are a powerful tool for DNA fragment analysis, offering significant advantages over agarose gels.
    • Despite preparation challenges, polyacrylamide gels yield superior results in resolution, capacity, and DNA purity.