Microbacterium algeriense sp. nov., a novel actinobacterium isolated from Algerian oil production waters

A non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i.e., strain G1^T) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3-3.5 % (w/v) NaCl, at 20-50 °C and at pH 6.0-9.0. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that strain G1^T belonged to the genus Microbacterium. Strain G1 ^T was closely related to Microbacterium oxydans (DSM 20578^T) and Microbacterium maritypicum (DSM 12512^T) with 99.8 % sequence similarity and to Microbacterium saperdae (DSM 20169^T) with 99.6 % sequence similarity. Strain G1 ^T contained MK9, MK10, MK11, MK12 and MK13 as respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol and glycolipid as the major polar lipids. The major cellular fatty acids were anteiso-C_15:0, iso-C_16:0 and anteiso-C_17:0. The estimated DNA G+C content was 69.57 mol% based on its draft genome sequence. Genome annotation of strain G1^T predicted the presence of 3511 genes, of which 3483 were protein-coding and 47 were tRNA genes. The DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) values between strain G1^T and M. oxydans (DSM 20578^T) and M. maritypicum (DSM 12512^T) were in both cases far below the respective species boundary thresholds (27.5 and 28.0 % for DDH; and 84.40 and 84.82% for ANI, respectively). Based on the data presented above, strain G1^T was considered to represent a novel species for which the name Microbacterium algeriense is proposed with the type strain G1^T (=DSM 109018^T=LMG 31276^T).