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Published on: January 10, 2018
The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and
Alicia M Churchill-Angus1, Svetlana E Sedelnikova1, Thomas H B Schofield1
1Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom.
Abstract:
Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.
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