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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Nov 27, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

High-Throughput DNA Isolation in Vegetable Crops for Genomics Applications.

Pasquale Tripodi¹, Giovanna Festa²

¹Council for Agricultural Research and Economics - Research Centre for Vegetable and Ornamental Crops (CREA-OF), Pontecagnano, SA, Italy. pasquale.tripodi@crea.gov.it.

Methods in Molecular Biology (Clifton, N.J.)

|December 2, 2020

Summary

This study presents a cost-effective, high-throughput DNA extraction protocol for 198 samples in 96-well plates. The method ensures high-quality DNA suitable for genomics applications in various vegetable species.

Keywords:

DNA extraction Genomic applications High-throughput Vegetable crops

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Last Updated: Nov 27, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Area of Science:

Molecular Biology
Genomics
Plant Science

Background:

High-quality DNA isolation is critical for molecular biology and genomics applications, including crop whole-genome sequencing and genotyping.
Efficiently screening large plant populations necessitates increased DNA extraction throughput at reduced costs.

Purpose of the Study:

To develop and validate a cost-effective, high-throughput DNA extraction protocol suitable for large-scale plant sample processing.
To provide a standardized method for isolating high-quality DNA for genomic applications in vegetable crops.

Main Methods:

A homemade DNA extraction protocol was developed and optimized for 96-well plate format.
The protocol enables the simultaneous isolation of DNA from 198 samples in a single extraction run.
Validation was performed on DNA extracted from tomato and pepper samples.

Main Results:

The protocol successfully isolates high-quality DNA, meeting precise standards for concentration and purity required for genomics.
Demonstrated high-throughput capability, processing 198 samples efficiently in a single 96-well plate extraction.
Validated efficacy across multiple vegetable species, including tomato and pepper.

Conclusions:

The developed protocol offers a scalable and affordable solution for DNA extraction in plant genomics.
This method significantly enhances throughput for screening large populations, supporting advancements in crop molecular breeding.
The protocol's adaptability across vegetable species makes it a valuable tool for diverse research applications.