Influence of particulate alkaline biomaterial remnants in dentin on the adhesion of two resin-based bonding systems

To study aimed to evaluate the influence of infiltrated alkaline biomaterials on the bond strength of composite obtained with etch and rinse and universal adhesive systems. Coronal dentin of extracted human molars was exposed and infiltrated with calcium hydroxide, MTA, or bioactive glass 45S5 powder. Non-infiltrated dentin specimens served as controls. Subsequently, dentin was either acid-etched or not, and remaining alkali content was measured by pH induction in water. Shear bond strength of an etch-and rinse system (Adper Single Bond 2) on etched dentin was then compared to that of a universal counterpart (Single Bond [(Scotchbond) Universal] by the same manufacturer. Data was compared between groups using one-way ANOVA and Tukey's HSD. On sound dentin, the etch-and-rinse system adhered stronger than the universal system (p < .05). All particles rendered the dentin alkaline. Acid etching reduced the alkali content in the infiltrated dentin. However, bond strength values were stronger with the universal system without etching than with the etch-and rinse counterpart, which lost more than 60% of its adhesiveness. The universal adhesive system was less susceptible to alkaline contamination of the dentin than the etch-and-rinse counterpart. Based on the similar composition of the two systems under investigation, this is likely to the due to its 10-MDP component.