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The Nucleosome Remodeling and Deacetylase Complex Has an Asymmetric, Dynamic, and Modular Architecture
Jason K K Low1, Ana P G Silva1, Mehdi Sharifi Tabar1
1School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
Cell Reports
|December 2, 2020
Summary
The nucleosome remodeling and deacetylase (NuRD) complex
Area of Science:
- Molecular Biology
- Epigenetics
- Structural Biology
Background:
- The nucleosome remodeling and deacetylase (NuRD) complex is crucial for metazoan development.
- Understanding NuRD's structure and function has been challenging due to its complexity.
Purpose of the Study:
- To define the architecture of the native mammalian NuRD complex.
- To elucidate the stoichiometry and regulatory mechanisms of NuRD.
Main Methods:
- Quantitative mass spectrometry
- Cross-linking mass spectrometry
- Protein biochemistry
- Electron microscopy
Main Results:
- The NuRD complex comprises distinct deacetylase (MTA, HDAC, RBBP) and remodeling (MBD, GATAD2, CHD) modules.
- GATAD2 regulates complex asymmetry and recruits the CHD remodeler.
- PWWP2A competes with MBD for binding, modulating NuRD composition.
Conclusions:
- This study resolves NuRD stoichiometry controversies and provides structural insights.
- A biochemical mechanism for PWWP2A-mediated regulation of NuRD is established.
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