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Bio-based and cost effective method for phenolic compounds removal using cross-linked enzyme aggregates.

Kheireddine Sellami1, Annabelle Couvert2, Noureddine Nasrallah3

  • 1Laboratoire de Génie de la Réaction, Faculté de Génie Mécanique et Génie des Procédés, Université des Sciences et de la Technologie Houari Boumediene, Bab Ezzouar, Alger 16111, Algeria; Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR 6226, F-35000 Rennes, France.

Journal of Hazardous Materials
|December 3, 2020
PubMed
Summary

This study presents a green wastewater treatment method using immobilized radish peroxidase (RSVNP-CLEAs). The novel enzyme preparation efficiently degrades phenol and p-cresol, showing enhanced stability and reusability.

Keywords:
CLEAsEnzymatic transformationGreen processPeroxidase immobilizationPhenols

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Area of Science:

  • Environmental Biotechnology
  • Enzyme Immobilization
  • Wastewater Treatment

Background:

  • Wastewater contamination by phenolic compounds poses environmental risks.
  • Developing stable and reusable enzymatic catalysts is crucial for effective bioremediation.
  • Raphanus sativus var. niger (RSVNP) peroxidase offers a potential new source for enzyme-based treatment.

Purpose of the Study:

  • To develop a green and efficient method for wastewater treatment using immobilized RSVNP.
  • To enhance the stability and reusability of RSVNP through cross-linked enzyme aggregates (CLEAs).
  • To evaluate the efficacy of RSVNP-CLEAs in degrading phenol and p-cresol.

Main Methods:

  • Immobilization of RSVNP as cross-linked enzyme aggregates (CLEAs) using acetone and glutaraldehyde.
  • Characterization of RSVNP-CLEAs for chemical, thermal, and storage stability.
  • Assessing the degradation efficiency of phenol and p-cresol under various operating conditions.
  • Evaluating the reusability of RSVNP-CLEAs over multiple treatment cycles.

Main Results:

  • RSVNP-CLEAs achieved high aggregation yield (85%) and recovered activity (>29%).
  • Immobilized enzyme exhibited superior chemical, thermal, and storage stability compared to free RSVNP.
  • RSVNP-CLEAs demonstrated high degradation efficiencies: 98% for p-cresol in 40 min and 92% for phenol in 1 hour.
  • The enzyme retained significant activity after multiple reuses, degrading 71% phenol and 54% p-cresol by the third and fourth batches, respectively.

Conclusions:

  • Immobilization of RSVNP as CLEAs provides a robust and stable enzymatic system for wastewater treatment.
  • RSVNP-CLEAs are effective in removing phenolic pollutants like phenol and p-cresol with high efficiency.
  • The reusability of RSVNP-CLEAs supports their potential for cost-effective and sustainable environmental applications.