Related Experiment Video
Updated: Nov 27, 2025
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
Published on: December 5, 2020
Suppression of Ovarian Cancer Cell Growth by AT-MSC Microvesicles
Agnieszka Szyposzynska1, Aleksandra Bielawska-Pohl1, Agnieszka Krawczenko1
1Laboratory of Biology of Stem and Neoplastic Cells, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland.
Abstract:
Transport of bioactive cargo of microvesicles (MVs) into target cells can affect their fate and behavior and change their microenvironment. We assessed the effect of MVs derived from human immortalized mesenchymal stem cells of adipose tissue-origin (HATMSC2-MVs) on the biological activity of the ovarian cancer cell lines ES-2 (clear cell carcinoma) and OAW-42 (cystadenocarcinoma). The HATMSC2-MVs were characterized using dynamic light scattering (DLS), transmission electron microscopy, and flow cytometry. The anti-tumor properties of HATMSC2-MVs were assessed using MTT for metabolic activity and flow cytometry for cell survival, cell cycle progression, and phenotype. The secretion profile of ovarian cancer cells was evaluated with a protein antibody array. Both cell lines internalized HATMSC2-MVs, which was associated with a decreased metabolic activity of cancer cells. HATMSC2-MVs exerted a pro-apoptotic and/or necrotic effect on ES-2 and OAW-42 cells and increased the expression of anti-tumor factors in both cell lines compared to control. In conclusion, we confirmed an effective transfer of HATMSC2-MVs into ovarian cancer cells that resulted in the inhibition of cell proliferation via different pathways, apoptosis and/or necrosis, which, with high likelihood, is related to the presence of different anti-tumor factors secreted by the ES-2 and OAW-42 cells.
Insights
Human adipose stem cell-derived microvesicles (HATMSC2-MVs) effectively target ovarian cancer cells. These MVs inhibit cancer cell proliferation through apoptosis and necrosis, offering potential anti-cancer therapeutic strategies.
Area of Science:
- Cell Biology
- Biotechnology
- Oncology
Background:
- Microvesicles (MVs) mediate intercellular communication by transporting bioactive cargo.
- Mesenchymal stem cells (MSCs) are explored for their therapeutic potential in cancer treatment.
- Ovarian cancer remains a significant health challenge, necessitating novel therapeutic approaches.
Purpose of the Study:
- To investigate the anti-tumor effects of microvesicles derived from human immortalized adipose tissue-origin mesenchymal stem cells (HATMSC2-MVs) on ovarian cancer cell lines.
- To determine the mechanisms by which HATMSC2-MVs influence ovarian cancer cell behavior, including proliferation, survival, and phenotype.
Main Methods:
- Characterization of HATMSC2-MVs using dynamic light scattering (DLS), transmission electron microscopy, and flow cytometry.
- Assessment of anti-tumor properties via MTT assays for metabolic activity and flow cytometry for cell survival, cell cycle progression, and phenotype.
- Evaluation of ovarian cancer cell secretion profiles using protein antibody arrays.
Main Results:
- Both ES-2 (clear cell carcinoma) and OAW-42 (cystadenocarcinoma) ovarian cancer cell lines internalized HATMSC2-MVs.
- HATMSC2-MV treatment led to decreased metabolic activity, increased apoptosis/necrosis, and altered cell cycle progression in cancer cells.
- HATMSC2-MVs enhanced the expression of anti-tumor factors in ovarian cancer cells.
Conclusions:
- HATMSC2-MVs are effectively transferred into ovarian cancer cells, inhibiting their proliferation.
- The anti-proliferative effect is mediated through apoptosis and/or necrosis, potentially linked to secreted anti-tumor factors.
- HATMSC2-MVs demonstrate significant potential as a therapeutic agent against ovarian cancer.
Related Concept Videos
01:19Mesenchymal Stem Cells
01:22MicroRNAs