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Long Intergenic Non-Protein Coding RNA 01089 Weakens Tumor Proliferation, Migration, and Invasion by Sponging

Dongdong Zhang1, Xingdong Cai2, Songwang Cai1

  • 1Department of Thoracic Surgery, The First Affiliated Hospital, Jinan University, Guangzhou 510632, People's Republic of China.

Cancer Management and Research
|December 3, 2020
PubMed
Abstract

Insights

Long non-coding RNA 1089 (LINC01089) suppresses non-small cell lung cancer (NSCLC) growth by inhibiting miR-3187-3p. This finding positions LINC01089 as a potential therapeutic target for NSCLC treatment.

Area of Science:

  • Molecular Biology
  • Oncology

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in non-small cell lung cancer (NSCLC) development and metastasis.
  • The specific role of long intergenic non-protein coding RNA 1089 (LINC01089) in NSCLC remains largely unknown.

Purpose of the Study:

  • To investigate the function and mechanism of LINC01089 in NSCLC cell proliferation, migration, and invasion.
  • To determine the relationship between LINC01089 expression and overall survival in NSCLC patients.

Main Methods:

  • Utilized GEPIA 2.0 and OncomiR database for expression analysis and survival correlation.
  • Employed starBase for miRNA target prediction and constructed cell models for LINC01089 and miR-3187-3p.
  • Performed qRT-PCR, MTS assays, and Transwell assays to evaluate gene expression, proliferation, migration, and invasion.

Main Results:

  • LINC01089 expression was significantly downregulated in NSCLC tissues and cells.
  • Overexpression of LINC01089 inhibited proliferation, migration, and invasion in NSCLC cell lines (A549, SK-MES-1).
  • Identified miR-3187-3p as a direct target of LINC01089; miR-3187-3p overexpression promoted NSCLC cell proliferation, migration, and invasion, counteracting LINC01089's effects.

Conclusions:

  • LINC01089 functions as a tumor suppressor in NSCLC by sponging miR-3187-3p.
  • LINC01089 represents a promising therapeutic target for NSCLC.
Keywords:
lncRNAlung cancermiRNAprognosis

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