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miR-152/TNS1 axis inhibits non-small cell lung cancer progression through Akt/mTOR/RhoA pathway

Jinjin Duan1, Li Wang1, Liqun Shang1

  • 1Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.

Bioscience Reports
|December 3, 2020
PubMed
Abstract

Insights

Tensin 1 (TNS1) promotes non-small cell lung cancer (NSCLC) growth and metastasis. The miR-152/TNS1 axis, via the Akt/mTOR/RhoA pathway, inhibits NSCLC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality worldwide.
  • Understanding the molecular mechanisms driving NSCLC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role and mechanism of tensin 1 (TNS1) in the progression of non-small cell lung cancer (NSCLC).
  • To elucidate the relationship between TNS1, miR-152, and the Akt/mTOR/RhoA signaling pathway in NSCLC.

Main Methods:

  • TNS1 expression was analyzed in NSCLC cells and tissues using RT-PCR and Western blot.
  • Cell proliferation, migration, and invasion were assessed using MTT, flow cytometry, wound healing, and Transwell assays.
  • The interaction between TNS1 and miR-152 was confirmed via luciferase assays, and the Akt/mTOR/RhoA pathway was evaluated.

Main Results:

  • TNS1 expression was significantly upregulated in NSCLC tissues and cells, correlating with poorer patient prognosis.
  • TNS1 overexpression promoted NSCLC cell growth, migration, and invasion.
  • TNS1 was identified as a direct target of miR-152, and the miR-152/TNS1 axis regulated NSCLC progression through the Akt/mTOR/RhoA pathway.

Conclusions:

  • The miR-152/TNS1 axis plays a critical role in inhibiting NSCLC progression.
  • Targeting the miR-152/TNS1 axis via the Akt/mTOR/RhoA pathway represents a potential therapeutic strategy for NSCLC.
Keywords:
Akt/mTOR/RhoA pathwayNSCLCTNS1growthmetastasismiR-152

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