Kineobactrum salinum sp. nov., isolated from marine sediment

Strain M2^T, isolated from marine sediment collected at Jeju Island, was an aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, rod-shaped bacterium that formed circular, raised, yellow colonies. Strain M2^T grew at 15-42 °C, pH 5.5-9.0 and with 1-9 % (w/v) NaCl. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain M2^T was closely related to Kineobactrum sediminis F02^T (98.0 % sequence similarity). Ubiquinone-8 was determined to be the sole respiratory quinone. Summed feature 3 (C_16 : 1  ω6c/C_16 : 1  ω7c) and summed feature 8 (C_18 : 1  ω7c/C_18 : 1  ω6c) were identified as the predominant fatty acids. The DNA G+C content and digital DNA-DNA relatedness between strain M2^T and K. sediminis F02^T were 60.7 mol% and 19.5 %, respectively. Phosphatidylglycerol and phosphatidylethanolamine were identified as the major polar lipids. Thus, polyphasic characterization revealed that strain M2^T represents a novel species in the genus Kineobactrum, for which the name Kineobactrum salinum sp. nov. is proposed. The type strain is M2^T (=KCTC 72815^T=VTCC 910108^T).