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Related Experiment Video

Updated: Nov 27, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

CLA4 regulates leaf angle through multiple hormone signaling pathways in maize.

Dandan Dou¹, Shengbo Han¹, Liru Cao²

¹College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.

Journal of Experimental Botany

|December 3, 2020

Summary

This summary is machine-generated.

ZmCLA4 acts as a transcriptional repressor controlling maize leaf angle by binding to specific DNA motifs. It integrates multiple plant hormone pathways, offering insights into crop architecture regulation.

Keywords:

DAP-Seq ZmCLA4 hormone signaling pathways leaf angle maize transcriptional repressor

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Published on: March 5, 2017

Area of Science:

Plant Biology
Genetics
Agronomy

Background:

Leaf angle is a key agronomic trait influencing crop architecture and yield in cereals.
The precise molecular mechanism by which ZmCLA4 regulates leaf angle in maize (Zea mays) is not fully understood.

Purpose of the Study:

To elucidate the function and regulatory mechanism of ZmCLA4 in controlling leaf angle in maize.
To identify the DNA binding motifs and target genes of ZmCLA4.

Main Methods:

Utilized the Gal4-LexA/UAS system and transactivation analysis to determine ZmCLA4's transcriptional activity.
Employed DNA affinity purification sequencing (DAP-Seq) to identify ZmCLA4 binding sites and motifs.
Analyzed ZmCLA4 target genes involved in plant hormone signaling pathways.

Main Results:

ZmCLA4 was confirmed as a transcriptional repressor regulating leaf angle in maize.
DAP-Seq identified ZmCLA4 binding to promoters with EAR (CACCGGAC), CCGARGS, and CDTCNTC motifs.
ZmCLA4 directly targets genes in auxin, brassinosteroid, abscisic acid, jasmonic acid, and ethylene signaling pathways.

Conclusions:

ZmCLA4 acts as a central regulatory hub integrating multiple plant hormone pathways to control maize leaf angle.
Understanding the ZmCLA4 regulatory network provides valuable insights for improving crop architecture and yield.