Enhanced Cytotoxicity of Cadmium by a Sulfated Polysaccharide from Abalone

Consumption of seafood is a common route of cadmium ion (Cd²⁺) exposure to consumers. The seafood matrices may alter the toxicity profile of Cd²⁺ due to the interaction between Cd²⁺ and biomacromolecules in seafood. In this study, enhanced cytotoxicity of Cd²⁺ was found in the presence of an abalone gonad sulfated polysaccharide (AGSP) and the mechanism was investigated at a metabolic level. The formation of the AGSP-Cd²⁺ complex was demonstrated by isothermal titration calorimetry. The level of reactive oxygen species (ROS) increased and mitochondrial membrane potential reduced upon exposure to the AGSP-Cd²⁺ complex as compared with those of Cd²⁺ exposure. The decreased cell viability after incubation with the AGSP-Cd²⁺ complex also suggested enhanced Cd²⁺ toxicity induced by AGSP. The metabolomics and lipidomics analysis revealed that, compared with the Cd²⁺ group, the AGSP-Cd²⁺ downregulated the phospholipid metabolism and resulted in more serious damage in the cellular membrane. The lipid metabolism disorder, in turn, amplified the generation of ROS, leading to a decrease in cell viability. These results provided new evidence of the enhanced Cd²⁺ toxicity upon interaction with seafood polysaccharides, and much attention should be paid to the effect of food ingredients on heavy metal ion toxicity.