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How bioaugmentation with Comamonas testosteroni accelerates pyridine mono-oxygenation and mineralization
Ge Zhu1, Yongming Zhang1, Songyun Chen1
1Department of Environmental Engineering, School of Environmental and Geographical Science, Shanghai Normal University, Shanghai, 200234, PR China.
Environmental Research
|December 3, 2020
Summary
Bioaugmentation with Comamonas testosteroni enhances pyridine biodegradation by accelerating initial mono-oxygenation. This synergy between the introduced bacteria and the existing microbial community improves overall pyridine removal and mineralization in wastewater.
Area of Science:
- Environmental microbiology
- Biotechnology
- Wastewater treatment
Background:
- Pyridine is a prevalent industrial wastewater contaminant.
- Biodegradation of pyridine is initiated by mono-oxygenation.
- Bioaugmentation is a strategy to enhance the removal of recalcitrant organic compounds.
Purpose of the Study:
- To investigate the specific role of bioaugmented bacteria in pyridine biodegradation.
- To understand the synergistic interactions between introduced and indigenous microbial communities.
- To optimize pyridine removal and mineralization in wastewater treatment.
Main Methods:
- Acclimation of activated-sludge biomass for pyridine biodegradation.
- Isolation of Comamonas testosteroni for its ability to degrade pyridine.
- Bioaugmentation of acclimated biomass with C. testosteroni.
- Analysis of pyridine removal, mineralization rates, and microbial community composition.
Main Results:
- Comamonas testosteroni individually removed pyridine faster than acclimated biomass, but with slower mineralization.
- Bioaugmentation with C. testosteroni accelerated both pyridine biodegradation and mineralization rates.
- C. testosteroni primarily enhanced the initial mono-oxygenation step, while the acclimated biomass facilitated downstream reactions.
- The bioaugmented microbial community showed increased C. testosteroni abundance with minimal impact on overall community structure.
Conclusions:
- Comamonas testosteroni plays a key role in accelerating the initial mono-oxygenation of pyridine.
- Synergistic interactions between bioaugmented C. testosteroni and the indigenous microbial community enhance pyridine biodegradation and mineralization.
- Bioaugmentation is an effective strategy for improving pyridine removal in industrial wastewater treatment through targeted microbial enhancement.
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