Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of flagellar architecture and metabolic pathways associated with the Leishmania mexicana SUMO protease.

Microbial pathogenesis·2026
Same author

A versatile 2A peptide-based strategy for ectopic expression and endogenous gene tagging in <i>Trypanosoma cruzi</i>.

Heliyon·2024
Same author

Antibodies to variable surface antigens induce antigenic variation in the intestinal parasite Giardia lamblia.

Nature communications·2023
Same author

Screening and Identification of Metacaspase Inhibitors: Evaluation of Inhibition Mechanism and Trypanocidal Activity.

Antimicrobial agents and chemotherapy·2020
Same author

Design, synthesis, and evaluation of substrate - analogue inhibitors of Trypanosoma cruzi ribose 5-phosphate isomerase type B.

Bioorganic & medicinal chemistry letters·2020
Same author

Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion with Caspases.

Molecular cell·2020

Related Experiment Video

Updated: Nov 27, 2025

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

8.2K

Update on relevant trypanosome peptidases: Validated targets and future challenges.

Vanina E Alvarez1, Paula A Iribarren1, Gabriela T Niemirowicz1

  • 1Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde", Universidad Nacional de San Martín - IIBIO, UNSAM-CONICET, San Martín, B1650HMP Buenos Aires, Argentina.

Biochimica Et Biophysica Acta. Proteins and Proteomics
|December 3, 2020
PubMed
Summary

Proteases like cruzipain and rhodesain are essential in parasites causing Chagas disease and sleeping sickness. New inhibitors targeting these parasite proteasomes offer promising therapeutic leads for neglected tropical diseases.

Keywords:
Antiparasitic agentChagas diseaseInhibitorPeptidaseProteaseSleeping sicknessTarget

More Related Videos

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor
08:50

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor

Published on: January 19, 2024

849
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
08:44

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Published on: January 22, 2019

7.6K

Related Experiment Videos

Last Updated: Nov 27, 2025

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

8.2K
Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor
08:50

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor

Published on: January 19, 2024

849
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
08:44

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Published on: January 22, 2019

7.6K

Area of Science:

  • Parasitology
  • Biochemistry
  • Drug Discovery

Background:

  • Trypanosoma cruzi causes Chagas disease, while Trypanosoma brucei gambiense and T. brucei rhodesiense cause Human African Trypanosomiasis (HAT).
  • These parasites possess various peptidases, including abundant cysteine proteases like cruzipain and rhodesain, vital for their survival.
  • These essential enzymes represent potential therapeutic targets for treating these neglected diseases.

Purpose of the Study:

  • To review the role of peptidases in Trypanosoma parasites.
  • To highlight proteases as drug targets for Chagas disease and HAT.
  • To discuss recent advancements in developing selective protease inhibitors.

Main Methods:

  • Literature review of characterized peptidases in Trypanosoma species.
  • Analysis of the abundance and essentiality of specific protease families.
  • Evaluation of recent findings on parasite-selective proteasome inhibitors.

Main Results:

  • Cysteine proteases (Clan CA), including Cathepsin L-like cruzipain and rhodesain, and Cathepsin B-like enzymes, are abundant and crucial in T. cruzi and T. brucei.
  • Several other characterized proteases also show potential as drug candidates.
  • Novel inhibitors with high selectivity for parasite proteasomes have been identified.

Conclusions:

  • Proteases are essential for Trypanosoma parasites, making them viable targets for chemotherapy.
  • Targeted inhibitors, particularly those acting on parasite proteasomes, represent promising lead compounds for new drug development against neglected trypanosomiases.