Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Rapid deoxyribonuclease test with methyl green.

B L Elder, I Trujillo, D J Blazevic

Journal of Clinical Microbiology

|September 1, 1977

Summary

A rapid 4-hour deoxyribonuclease test using methyl green accurately differentiates Serratia species from other Enterobacteriaceae. This method showed 100% agreement with traditional overnight tests, offering a faster diagnostic alternative.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hippocampal Pallium Lesion Impairs Transitive Inference in Goldfish.

Hippocampus·2025

Same author

Three-dimensional evaluation of postoperative stability: a comparative study between surgery-first and surgery-late protocols.

International journal of oral and maxillofacial surgery·2022

Same author

Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations.

Genome·2007

Same author

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2003

Same author

DNA probe for beta-hemolytic group B Streptococcus. Diagnostic accuracy in threatened preterm labor.

The Journal of reproductive medicine·1999

Same author

How to protect confidentiality during the contracting process.

QRC advisor·1995

Area of Science:

Microbiology
Clinical Diagnostics
Bacterial Identification

Background:

Accurate identification of Enterobacteriaceae is crucial for effective patient treatment.
Distinguishing Serratia species from other related bacteria can be challenging with standard methods.
Current identification methods often require prolonged incubation times.

Purpose of the Study:

To develop and validate a rapid deoxyribonuclease (DNase) test for differentiating Serratia species.
To compare the efficacy of the new 4-hour DNase test against established overnight culture methods.
To assess the test's performance across various Enterobacteriaceae species.

Main Methods:

A 4-hour deoxyribonuclease test was developed utilizing methyl green as an indicator.
The test was performed on pure cultures of Serratia, Enterobacter, and Klebsiella species.
Results were compared with those obtained from a standard overnight plate test.

Main Results:

The 4-hour deoxyribonuclease test demonstrated 100% agreement with the overnight plate test.
This high concordance was observed across 100 Serratia, 83 Enterobacter, and 6 Klebsiella species tested.
The test effectively differentiated Serratia from the other tested Enterobacteriaceae.

Conclusions:

The developed 4-hour deoxyribonuclease test is a reliable and rapid method for identifying Serratia species.
This assay offers a significant time-saving advantage over conventional overnight identification techniques.
The methyl green-based DNase test provides a valuable tool for clinical microbiology laboratories.

Related Experiment Videos