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Related Experiment Video

Updated: Nov 27, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

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An optimized base editor with efficient C-to-T base editing in zebrafish.

Yu Zhao1, Dantong Shang1, Ruhong Ying1

  • 1Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

BMC Biology
|December 4, 2020
PubMed
Summary

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A new base editor, zAncBE4max, improves CRISPR base editing efficiency in zebrafish. This tool precisely models human diseases by correcting specific mutations, overcoming limitations of previous methods.

Area of Science:

  • Genetics
  • Molecular Biology
  • Zebrafish Models

Background:

  • Zebrafish are crucial model organisms for studying gene function and human diseases due to genetic homology.
  • CRISPR/Cas9 base editors enable targeted single-base mutations but show lower efficiency and higher indel formation in zebrafish compared to other models.

Purpose of the Study:

  • To develop a more efficient and accurate base editor for zebrafish.
  • To enhance the utility of zebrafish as a model for human genetic diseases.

Main Methods:

  • Designed and constructed a novel base editor (zAncBE4max) optimized for zebrafish codon preference.
  • Tested the editor's efficiency and specificity at multiple target sites in zebrafish.

Main Results:

Keywords:
BE4maxBase editorCRISPR/Cas9Zebrafish

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Last Updated: Nov 27, 2025

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  • The zAncBE4max system demonstrated high efficiency in inducing C-to-T base substitutions with minimal indel formation.
  • Successfully modeled the Twist2 p.E78K mutation in zebrafish, replicating features of human ablepharon macrostomia syndrome (AMS).

Conclusions:

  • zAncBE4max represents a significant advancement for precise base editing in zebrafish.
  • This tool enhances the capability of zebrafish models for studying and understanding human genetic disorders.