Related Experiment Video
Updated: Nov 27, 2025
10:26
TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
Published on: September 10, 2015
18.1K
Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq
Lisa C Lyons1,2, Snehajyoti Chatterjee3, Yann Vanrobaeys3
1Department of Neuroscience and Pharmacology, Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. lyons@bio.fsu.edu.
Molecular Brain
|December 4, 2020
Summary
Sleep deprivation alters actively translated genes in the brain, impacting neuronal function. This study reveals significant differences between changes in the transcriptome and translatome following sleep loss.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Sleep deprivation is a prevalent global health issue with detrimental effects on cognitive functions and overall health.
- While transcriptional changes in the brain due to sleep deprivation are documented, the impact on post-transcriptional gene regulation, specifically the translatome, remains less understood.
- The hippocampus is particularly vulnerable to acute sleep deprivation, suggesting region-specific effects on gene regulation.
Purpose of the Study:
- To investigate the effects of sleep deprivation on the translatome in excitatory neurons.
- To compare changes in actively translated mRNAs (translatome) with overall gene expression (transcriptome) in the hippocampus after sleep deprivation.
Main Methods:
- Utilized the RiboTag mouse model to specifically isolate and sequence ribosome-associated mRNAs from CaMKIIα-expressing excitatory neurons.
- Expressed HA-labeled Rpl22 in these neurons to tag actively translated transcripts.
- Analyzed differentially expressed genes in the translatome post-sleep deprivation and compared them with existing transcriptome data.
Main Results:
- Identified 198 differentially expressed genes in the translatome of excitatory neurons following sleep deprivation.
- Found significant divergence between translatome and transcriptome changes, with only 49 genes showing similar regulation.
- Observed 478 genes differentially regulated in the transcriptome but not the translatome, and 149 genes regulated in the translatome but not the transcriptome.
- Pathway analysis indicated distinct biological functions for genes uniquely regulated in either the transcriptome or translatome.
Conclusions:
- Sleep deprivation significantly alters the pool of actively translated mRNAs in excitatory neurons.
- Post-transcriptional regulation plays a crucial role in the brain's response to sleep loss, with distinct functional pathways affected.
- Understanding translatome changes provides a more nuanced view of sleep deprivation's molecular impact on neuronal function.
Related Concept Videos
01:29Translational Regulation
386
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
386
02:24Ribosome Profiling
3.9K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.9K
02:28Leaky Scanning
5.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K