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Genetically engineered antibody molecules and their application.

S L Morrison1, L Wims, S Wallick

  • 1Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032.

Annals of the New York Academy of Sciences
|January 1, 1987
PubMed
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Researchers engineered novel antibody molecules by manipulating immunoglobulin genes for enhanced expression in myeloma cells. This genetic engineering enables the creation of unique antibodies for research and potential therapeutic applications in human diseases.

Area of Science:

  • Molecular Biology
  • Immunology
  • Biotechnology

Background:

  • Immunoglobulin gene expression is crucial for antibody production.
  • Myeloma cells offer an efficient system for gene expression studies.
  • Genetic manipulation of antibodies can lead to novel functionalities.

Purpose of the Study:

  • To develop methods for efficient immunoglobulin gene expression in myeloma cells.
  • To engineer novel antibody molecules with altered specificities and structures.
  • To explore the potential of genetically engineered antibodies in research and therapy.

Main Methods:

  • Utilizing protoplast fusion for high-frequency transfection of myeloma cells.
  • Employing compatible plasmids with dual selectable markers for simultaneous gene delivery.

Related Experiment Videos

  • Cloning rearranged variable regions from hybridomas or cDNAs for antibody engineering.

    • Main Results:

    • Achieved transfection frequencies greater than 10(-3) for immunoglobulin genes.
    • Successfully produced antibodies with combined variable regions from different sources (e.g., mouse and human).
    • Generated antibodies with modified constant regions, creating unique molecular reagents.

    Conclusions:

    • Genetic engineering of immunoglobulin genes provides a powerful tool for creating novel antibody molecules.
    • These engineered antibodies serve as valuable reagents for studying antibody structure-function relationships.
    • The developed techniques hold potential for the diagnosis and therapy of human diseases.