Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the NAT10 acetyltransferase and mechanism of tRNA acetylation.

Nature communications·2026
Same author

Hierarchical small molecule inhibition of MYST acetyltransferases.

Nature communications·2026
Same author

Seven wonders of RNA modification biology.

RNA (New York, N.Y.)·2026
Same author

Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome.

Cell·2025
Same author

A sequence-specific RNA acetylation catalyst.

bioRxiv : the preprint server for biology·2025
Same author

Hierarchical small molecule inhibition of MYST acetyltransferases.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Nov 27, 2025

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.5K

Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting.

Keri M Bryson1, Supuni Thalalla-Gamage1, Jordan L Meier1

  • 1Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.

Current Protocols in Chemical Biology
|December 4, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel dot and immuno-northern blotting method to detect N4-acetylcytidine (ac4C) levels and distribution in RNA. This protocol enables efficient analysis of cytidine acetyltransferase activity in biological samples.

Keywords:
RNAac4Cacetylationcytidineepitranscriptomemodification

More Related Videos

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.4K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.5K

Related Experiment Videos

Last Updated: Nov 27, 2025

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.5K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.4K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • RNA Modifications

Background:

  • Cytidine acetyltransferases are emerging enzymes catalyzing N4-acetylcytidine (ac4C) formation in RNA.
  • Limited methods exist for assessing cytidine acetyltransferase activity and ac4C distribution in biological samples.

Purpose of the Study:

  • To develop and present a protocol for analyzing cellular cytidine acetyltransferase activity.
  • To enable the detection and visualization of ac4C across various RNA species.

Main Methods:

  • Utilizes dot blotting for detecting N4-acetylcytidine in RNA.
  • Employs immuno-northern blotting for visualizing N4-acetylcytidine distribution in RNA.
  • Describes protocols for analyzing cellular cytidine acetyltransferase activity.

Main Results:

  • Successfully established dot and immuno-northern blotting techniques for ac4C detection.
  • Provides a method to assess enzyme activity and RNA modification distribution.
  • Offers a protocol for analyzing cytidine acetyltransferase activity in biological samples.

Conclusions:

  • The described dot and immuno-northern blotting methods offer a rapid approach to study ac4C.
  • This protocol facilitates the assessment of cytidine acetyltransferase activity and ac4C profiling.
  • Enables deeper investigation into the biological roles of ac4C RNA modifications.