BC@DNA-Mn₃(PO₄)₂ Nanozyme for Real-Time Detection of Superoxide from Living Cells

Electrochemical in situ sensing of small signal molecules released from living cells has an increasing significance in early diagnosis, pathological analyses, and drug discovery. Here, a living cell-fixed sensing platform was built using the BC@DNA-Mn₃(PO₄)₂ nanozyme, in which a highly biocompatible bacterial cellulose riveted with very tiny Mn₃(PO₄)₂; it not only delivers high catalytic activity toward superoxide anions but possesses excellent biocompatibility for cell adsorption and growth. Additionally, the experimental results suggested that fixing the living cells on the surface of the sensing platform facilitates tiny Mn₃(PO₄)₂ activity centers to capture and detect O₂^•- very quickly and simultaneously has great potential in miniaturization, cost reduction, and real-time monitoring.