Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Operons02:09

Operons

53.1K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
53.1K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.7K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individual differences in prosocial learning are represented in the hippocampal dorsal CA1.

Nature neuroscience·2026
Same author

Finasteride withdrawal induces anxiety-like behavior and novelty avoidance in adult male rats.

Journal of neuroendocrinology·2026
Same author

Cyclase-associated protein 2 gene delivery: A potential multi-target approach for preventing synaptic failure in Alzheimer's disease.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Oxytocin seals the blood-brain barrier, improving 22q11.2 deletion syndrome trajectories.

Brain : a journal of neurology·2025
Same author

What's in your plate?AMPA receptors modulation at the crossroads of homeostatic and hedonic eating.

Pharmacological research·2025
Same author

Synaptic-dependent developmental dysconnectivity in 22q11.2 deletion syndrome.

Science advances·2025
Same journal

Simultaneous Ca<sup>2+</sup> Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices.

Current protocols in neuroscience·2020
Same journal

A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.

Current protocols in neuroscience·2020
Same journal

Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Recombinant Antibodies in Basic Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.

Current protocols in neuroscience·2020
Same journal

Whole-Brain Image Analysis and Anatomical Atlas 3D Generation Using MagellanMapper.

Current protocols in neuroscience·2020
See all related articles

Related Experiment Video

Updated: Nov 27, 2025

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

440

Automated Two-Chamber Operon ID/ED Task for Mice.

Francesca Scarsi1, Diego Scheggia1, Francesco Papaleo1

  • 1Genetics of Cognition Laboratory, Neuroscience Area, Istituto Italiano di Tecnologia, Genova, Italy.

Current Protocols in Neuroscience
|December 4, 2020
PubMed
Summary
This summary is machine-generated.

This study details an upgraded automated Operon ID/ED task for mice, enhancing cognitive flexibility research. The improved system aids in preclinical drug testing and genetic screening for conditions like schizophrenia.

Keywords:
attentionautomated taskcognitive flexibilityexecutive functionsmicereversalset-shifting

More Related Videos

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
10:49

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Published on: February 3, 2023

3.3K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

3.6K

Related Experiment Videos

Last Updated: Nov 27, 2025

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

440
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
10:49

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Published on: February 3, 2023

3.3K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

3.6K

Area of Science:

  • Neuroscience
  • Cognitive Science
  • Animal Behavior

Background:

  • Attentional set shifting assesses cognitive flexibility and executive functions.
  • The Wisconsin Card Sorting Test (WCST) and CANTAB Intra-/Extra-Dimensional set-shifting task (ID/ED) are standard human assessments.
  • The automated Operon ID/ED task is a valuable preclinical tool for drug testing and genetic screening.

Purpose of the Study:

  • To describe an upgraded, commercially available automated two-chamber Operon ID/ED task for mice.
  • To provide a detailed protocol for using this upgraded task to study attentional set shifting.
  • To offer guidance on using the associated ANY-maze software for adaptability.

Main Methods:

  • Utilized an upgraded automated two-chamber Operon ID/ED system for mouse behavioral testing.
  • Employed a detailed step-by-step protocol for mouse preparation and testing.
  • Included a manual for the ANY-maze software to facilitate task adaptation.

Main Results:

  • The upgraded Operon ID/ED task allows for the study of attentional set shifting across two or three dimensions (lights, odors, textures).
  • A comprehensive guideline for operating the equipment and paradigm is provided.
  • The system is designed for effective preclinical drug testing and genetic screening.

Conclusions:

  • The upgraded Operon ID/ED task offers a robust and adaptable preclinical model for cognitive flexibility research.
  • This tool has direct translational relevance for understanding human cognitive disorders like schizophrenia.
  • The provided protocol and software manual ensure comprehensive usability for researchers.