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The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth
1Department of Genetics, Hebrew University, Jerusalem, Israel.
Current Genetics
|January 1, 1986
Abstract:
The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.