Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preferential recombination between GC clusters in yeast mitochondrial DNA.

C L Dieckmann1, B Gandy

  • 1Department of Biochemistry, University of Arizona, Tucson 85721.

The EMBO Journal
|December 20, 1987
PubMed
Summary

GC clusters in yeast mitochondrial DNA are preferred sites for recombination. This study reveals GC clusters facilitate intermolecular recombination, creating new DNA molecule families and highlighting their role as hotspots.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the early conception factor (ECF) test for the detection of nonpregnancy in dairy cattle.

Theriogenology·2001
Same author

Characterization of the EYE2 gene required for eyespot assembly in Chlamydomonas reinhardtii.

Genetics·2001
Same author

Eyespot-assembly mutants in Chlamydomonas reinhardtii.

Genetics·1999
Same author

Suppressor analysis of mutations in the 5'-untranslated region of COB mRNA identifies components of general pathways for mitochondrial mRNA processing and decay in Saccharomyces cerevisiae.

Genetics·1999
Same author

Regulation of poly(A) site choice of several yeast mRNAs.

Nucleic acids research·1998
Same author

Genetic evidence for interaction between Cbp1 and specific nucleotides in the 5' untranslated region of mitochondrial cytochrome b mRNA in Saccharomyces cerevisiae.

Molecular and cellular biology·1997

Area of Science:

  • Mitochondrial Genetics
  • Molecular Biology
  • Yeast Genomics

Background:

  • Yeast mitochondrial DNA features AT-rich spacers with GC clusters.
  • GC-rich elements are known sites for intramolecular recombination, forming petite molecules.

Purpose of the Study:

  • To investigate the role of GC clusters in intermolecular recombination between petite and grande yeast mitochondrial DNA.
  • To identify preferred sites for recombination events in yeast mitochondrial genomes.

Main Methods:

  • Utilized genetic selection to promote integration of petite mitochondrial DNA into the grande genome.
  • Analyzed recombination events, including crossover sites within homologous and non-homologous regions.

Main Results:

Related Experiment Videos

  • Identified GC clusters as favored sites for intermolecular recombination.
  • Demonstrated a novel recombination event where a 44 bp GC cluster in petite DNA exchanged with a homologous GC cluster in grande DNA.
  • Observed secondary recombination events leading to diverse mitochondrial DNA molecules with varying petite units.

    • Conclusions:

    • GC clusters act as preferred sites for intermolecular recombination in yeast mitochondrial DNA.
    • These findings support the role of GC clusters as recombinational hotspots, influencing petite excision and mitochondrial genome dynamics.