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Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Updated: Nov 27, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Glycoproteomics: growing up fast.

David R Thomas¹, Nichollas E Scott¹

¹Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Melbourne 3000, Australia.

Current Opinion in Structural Biology

|December 5, 2020

Summary

This summary is machine-generated.

Glycoproteomics advancements enable large-scale identification of glycosylation sites. New technologies and analysis strategies are driving the field towards studying disease mechanisms and specific biological processes.

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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

Area of Science:

Biochemistry
Proteomics
Analytical Chemistry

Background:

Glycoproteomics is crucial for understanding biological processes.
Significant efforts have focused on improving technologies for glycosylation analysis.
Current methods allow for large-scale identification of glycosylation sites.

Purpose of the Study:

To review recent developments in glycoproteomics.
To discuss state-of-the-art approaches for analyzing glycosylated substrates.
To highlight emerging trends in the field.

Main Methods:

Review of recent technological advancements in glycoproteomics.
Discussion of improved enrichment strategies for various glycosylation types.
Integration of mass spectrometry advancements for site identification and quantification.

Main Results:

Significant improvements in mass spectrometry instrumentation.
Enhanced enrichment approaches for mucin-O-glycosylation, O-GlcNAc glycosylation, and N-linked glycosylation.
Identification and quantification of hundreds to thousands of glycosylation sites per experiment.

Conclusions:

Glycoproteomics is rapidly advancing with new technologies.
Current approaches enable comprehensive analysis of glycosylation.
The field is shifting towards mechanistic and disease-specific studies.