Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CytokineProfile: An Integrated Web Tool for Cytokine Profiling Analysis.

Computational and structural biotechnology journal·2026
Same author

Salivary Biological Responses to Nonsurgical Periodontal Treatment in Type 2 Diabetics.

JDR clinical and translational research·2026
Same author

Realizing the clinical utility of saliva for monitoring oral diseases.

Periodontology 2000·2024
Same author

Fasting and Glucose Metabolism Differentially Impact Peripheral Inflammation in Human Type 2 Diabetes.

Nutrients·2024
Same author

RespirAnalyzer: an R package for analyzing data from continuous monitoring of respiratory signals.

Bioinformatics advances·2024
Same author

Comprehensive Analysis of Tumor Microenvironment Reveals Prognostic ceRNA Network Related to Immune Infiltration in Sarcoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2023

Related Experiment Video

Updated: Nov 27, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.8K

Long noncoding RNAs implicated in embryonic development in Ybx1 knockout zebrafish.

Chen Huang1, Bo Zhu2, Dongliang Leng2

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, China.

FEBS Open Bio
|December 5, 2020
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) influence Y-box-binding protein 1 (Ybx1) in zebrafish development. Disrupting Ybx1 affects lncRNAs that regulate key genes, impacting embryo morphology.

Keywords:
REDOXYBX1deep RNA sequencinglong noncoding RNAszebrafish embryo development

More Related Videos

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
12:50

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

Published on: March 31, 2011

22.0K
Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.0K

Related Experiment Videos

Last Updated: Nov 27, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.8K
Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
12:50

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

Published on: March 31, 2011

22.0K
Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.0K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genomics

Background:

  • Y-box-binding protein 1 (Ybx1) is crucial for vertebrate embryogenesis, reproduction, and development.
  • The role of long noncoding RNAs (lncRNAs) in Ybx1-mediated developmental processes is not fully understood.

Purpose of the Study:

  • To investigate the involvement of lncRNAs in Ybx1-regulated vertebrate development.
  • To identify lncRNAs affected by Ybx1 disruption during zebrafish embryogenesis.

Main Methods:

  • Systematic transcriptome analysis using RNA sequencing on Ybx1-deficient and wild-type zebrafish embryos.
  • Identification of lncRNAs potentially targeting reduction-oxidation-related genes (e.g., duox, noxo1a).
  • lncRNA knockdown experiments to assess effects on larval morphology.

Main Results:

  • Several lncRNAs were identified as being affected by Ybx1 disruption.
  • These lncRNAs may regulate genes involved in reduction-oxidation processes.
  • Knockdown of specific lncRNAs resulted in significant morphological deformities in zebrafish larvae.

Conclusions:

  • lncRNAs play a role in Ybx1-mediated zebrafish development.
  • Ybx1 influences lncRNAs that regulate critical developmental genes.
  • This study offers new insights into lncRNA-based mechanisms in Ybx1-deficient embryos.