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Updated: Nov 27, 2025
Author Spotlight: An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils
Published on: April 7, 2023
A novel nanohybrid antimicrobial based on chitosan nanoparticles and antimicrobial peptide microcin J25 with low
Haitao Yu1, Zhengxin Ma2, Shanyu Meng3
1Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, United States; Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, United States; Ministry of Agriculture and Rural Affairs Feed Industry Centre, China Agricultural University, Beijing, 100193, PR China.
Abstract:
Bacterial resistance to antibiotics is a critical public health concern. Alternatives of antibiotics are needed urgently. Herein, we designed and engineered a new nano-antimicrobial, chitosan nanoparticles (CNs)-antimicrobial peptide microcin J25 (MccJ25) conjugates (CNMs). The engineered CNMs proved to be highly active against Gram-negative and Gram-positive bacteria, and the activity of CNMs and CNs was stable in various thermal and pH environments. Escherichia coli K88 strain treated with CNMs did not acquire resistance in serial passage assays over a period of 18 days. Risk assessment with cell lines showed that CNMs did not cause toxicity. Additionally, CNMs did not reduce the lifespan of C. elegans. In summary, this study demonstrated that CNMs can serve as an excellent novel antimicrobial agent against multi-drug resistance pathogens.
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