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Rat jejunal disaccharidase activity increases biphasically during early post-natal development
1A.F.R.C. Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
Insights
Hydrocortisone injection in young rats accelerates sucrase appearance in the jejunum, mimicking normal development. This suggests complex control mechanisms for alpha-glucosidase induction during development.
Area of Science:
- Biochemistry
- Developmental Biology
- Gastroenterology
Background:
- Disaccharidase development in the rat jejunum is crucial for nutrient absorption.
- Steroid hormones are thought to play a role in mimicking developmental changes at weaning.
Purpose of the Study:
- To investigate the effect of hydrocortisone injection on jejunal disaccharidase appearance in young rats.
- To compare the enzyme induction patterns with normal developmental processes.
Main Methods:
- Injection of hydrocortisone into 9-day-old rats.
- Cytochemical analysis of disaccharidase (alpha-glucosidase) activity in jejunal enterocytes.
- Measurement of enterocyte migration rates and crypt depth.
Main Results:
- Hydrocortisone accelerated the appearance of sucrase and other alpha-glucosidases, with similar time courses to normal development.
- Enzyme activity initiated at the villus base and spread upwards.
- Enterocyte migration rates were similar between hydrocortisone-treated and early normal development phases.
- The rate of alpha-glucosidase increase in enterocytes was similar after hydrocortisone treatment and in early normal development, but increased significantly later in normal development.
Conclusions:
- Hydrocortisone injection can precociously induce jejunal disaccharidase activity in rats.
- The regulation of alpha-glucosidase induction during normal development is more complex than previously assumed.
- Cell migration rates differ between early and late phases of normal development, suggesting distinct regulatory mechanisms.
Abstract:
1. Injection of hydrocortisone into 9-day-old rats induces the early appearance of sucrase in jejunal homogenates, the time course of the subsequent increase and magnitude of the final effect being similar to that seen to start on day 16 during normal development. 2. Cytochemical comparison of the effect of hydrocortisone and normal development on the appearance of a mixture of sucrase, maltase, isomaltase and trehalase disaccharidases (alpha-glucosidase activity) shows this enzyme to appear first in enterocytes at the base of the villus. Enzyme activity then increases and spreads along the whole villus during the next 96 h. 3. The rate at which enterocytes migrate along the villus after hydrocortisone injection is not significantly different from that measured during the early phase of normal development. The later phase of normal development is associated with a threefold increase in cell migration rate and a twofold increase in crypt depth. 4. The rate at which alpha-glucosidase activity increases in enterocytes at the base of the villus during early normal development is similar to that determined after hydrocortisone injection into younger animals. This rate of appearance increases eight to tenfold during normal development, shortly after the appearance of solid food in the stomach of normal control animals. 5. Injection of steroid hormones into young rats is generally supposed to mimic events taking place normally at weaning. Present results show alpha-glucosidase induction during normal development to be under more complicated control than had been previously suspected.