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Related Experiment Videos

Rat jejunal disaccharidase activity increases biphasically during early post-natal development.

E K Lund1, M W Smith

  • 1A.F.R.C. Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.

The Journal of Physiology
|October 1, 1987
PubMed
Summary

Hydrocortisone injection in young rats accelerates sucrase appearance in the jejunum, mimicking normal development. This suggests complex control mechanisms for alpha-glucosidase induction during development.

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Gastroenterology

Background:

  • Disaccharidase development in the rat jejunum is crucial for nutrient absorption.
  • Steroid hormones are thought to play a role in mimicking developmental changes at weaning.

Purpose of the Study:

  • To investigate the effect of hydrocortisone injection on jejunal disaccharidase appearance in young rats.
  • To compare the enzyme induction patterns with normal developmental processes.

Main Methods:

  • Injection of hydrocortisone into 9-day-old rats.
  • Cytochemical analysis of disaccharidase (alpha-glucosidase) activity in jejunal enterocytes.
  • Measurement of enterocyte migration rates and crypt depth.

Main Results:

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  • Hydrocortisone accelerated the appearance of sucrase and other alpha-glucosidases, with similar time courses to normal development.
  • Enzyme activity initiated at the villus base and spread upwards.
  • Enterocyte migration rates were similar between hydrocortisone-treated and early normal development phases.
  • The rate of alpha-glucosidase increase in enterocytes was similar after hydrocortisone treatment and in early normal development, but increased significantly later in normal development.

Conclusions:

  • Hydrocortisone injection can precociously induce jejunal disaccharidase activity in rats.
  • The regulation of alpha-glucosidase induction during normal development is more complex than previously assumed.
  • Cell migration rates differ between early and late phases of normal development, suggesting distinct regulatory mechanisms.