Related Experiment Video
Updated: Nov 27, 2025
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
Published on: May 9, 2025
Melatonin induction of HSP90 expression exerts cryoprotective effect on ovarian tissue
Lu Yang Cheng1, Tie Cheng Sun2, Xiao Chao Liu3
1Department of Pathogenic Biology, School of Basic Medical Science, China Medical University, Shenyang, 110001, China; Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China.
Abstract:
Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT at 0, 0.001, 0.01, 0.1, and 1 mM, respectively. After cryopreservation-thawing, HSP90 mRNA and protein level were evaluated using qRT-PCR and western blot. The qRT-PCR results revealed that HSP90 mRNA expression was significantly (p < 0.01) upregulated in MLT-treated groups in comparison with the controls (0 mM). Western blot revealed higher HSP90 protein expression in MLT-treated groups compared to control group (0 mM), thus further confirming that MLT positively affected HSP90 expression. Moreover, 0.1 mM MLT had better effects than other concentrations of MLT. Conclusively, findings in the present work provide a feasible technology for improving cryopreserved ovarian tissue quality through the addition of MLT to elicit HSP90 expression.
Insights
Melatonin (MLT) enhances ovarian tissue cryopreservation by increasing Heat Shock Protein 90 (HSP90) expression. This study confirms MLT
Area of Science:
- Reproductive Biology
- Cryobiology
- Molecular Biology
Background:
- Previous research indicated melatonin (MLT) preserves cryopreserved ovarian tissue quality in mice.
- The specific mechanism by which MLT confers cryoprotection requires further elucidation.
Purpose of the Study:
- To investigate the role of melatonin (MLT) in upregulating Heat Shock Protein 90 (HSP90) expression within ovarian tissues.
- To determine if MLT-induced HSP90 expression contributes to the cryoprotective effects observed in ovarian tissues.
Main Methods:
- Ovarian tissues were collected from 50 female rats.
- Tissues were treated with varying concentrations of MLT (0, 0.001, 0.01, 0.1, and 1 mM).
- HSP90 mRNA and protein levels were quantified post-cryopreservation and thawing using qRT-PCR and Western blot analysis.
Main Results:
- Melatonin treatment significantly upregulated HSP90 mRNA expression compared to controls (p < 0.01).
- Western blot analysis confirmed elevated HSP90 protein levels in MLT-treated groups.
- The 0.1 mM MLT concentration demonstrated the most significant positive effect on HSP90 expression.
Conclusions:
- Melatonin effectively enhances HSP90 expression in cryopreserved ovarian tissues.
- Increased HSP90 expression mediated by MLT is a key factor in improving ovarian tissue cryoprotection.
- The addition of MLT presents a viable strategy for improving the quality of cryopreserved ovarian tissues.
More Related Videos
05:53Author Spotlight: Advancing Fertility Preservation in Young Female Cancer Patients Through Ovarian Tissue Vitrification and In Vitro Follicular Growth
Published on: August 9, 2024
12:02Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016