Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 27, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.2K

A Microfluidic Device for Modulation of Organellar Heterogeneity in Live Single Cells.

Ken-Ichi Wada1,2, Kazuo Hosokawa3, Yoshihiro Ito4

  • 1Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. wada.kenichi.833@m.kyushu-u.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|December 7, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Is it possible to generate artificial mitochondrial genome?]

Nihon yakurigaku zasshi. Folia pharmacologica Japonica·2026
Same author

Copying a soft lithography master mold using an inexpensive, hobby-use UV-curable resin.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Nucleic acid detection based on single-cluster analysis of cross-linking aggregates of DNA-modified gold nanoparticles using a dark-field microscope.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic device.

STAR protocols·2025
Same author

Density and structure of DNA immobilised on gold nanoparticles affect sensitivity in nucleic acid detection.

Scientific reports·2025

This study demonstrates quantitative control of peroxisome transfer between single cells using a microfluidic device. The device enables controlled organellar transfer, aiding research into cellular phenotypes and heterogeneity.

Area of Science:

  • Cell Biology
  • Microfluidics
  • Organelle Biology

Background:

  • Quantitative organellar transfer between single cells is crucial for studying cellular phenotypes.
  • Previous microfluidic devices enabled controlled mitochondrial transfer but not for other organelles.

Purpose of the Study:

  • To investigate the quantitative properties of peroxisome transfer using a microfluidic device.
  • To assess the device's applicability for controlled transfer of organelles beyond mitochondria.

Main Methods:

  • Utilized a microfluidic device to create strictured cytoplasmic connections between fused single cells.
  • Employed a Sendai virus envelope-based method for cell fusion.
  • Investigated peroxisome transfer through microtunnels of varying lengths (10 μm and 4 μm).
Keywords:
Microfluidic devicecell fusionmitochondriaorganellar transferperoxisome

More Related Videos

A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

9.0K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.4K

Related Experiment Videos

Last Updated: Nov 27, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.2K
A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

9.0K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.4K

Main Results:

  • Achieved strictured cytoplasmic connections with lengths matching the microtunnels (10 μm and 4 μm).
  • Observed a smaller number of peroxisomes transferred through the 10 μm microtunnel compared to the 4 μm microtunnel.
  • Demonstrated that the microfluidic device allows for quantitative control over peroxisome transfer.

Conclusions:

  • The microfluidic device successfully enables quantitatively controlled peroxisome transfer between single cells.
  • This platform can be extended to study the role of peroxisome heterogeneity in cellular phenotypes.
  • The findings support the device's potential for broader applications in organelle transfer research.