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Published on: January 28, 2020
Long non-coding RNA expression profiles in peripheral blood mononuclear cells of patients with coronary artery
Wei Zhong1,2,3, Qiaoting Deng2,3,4, Xunwei Deng2,3,4
1Center for Cardiovascular Diseases, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, China.
Insights
Long non-coding RNAs (lncRNAs) show altered expression in peripheral blood mononuclear cells (PBMC) of coronary artery disease (CAD) patients. These lncRNAs may serve as potential biomarkers for early detection of cardiac abnormalities.
Area of Science:
- Molecular Biology
- Genomics
- Cardiovascular Research
Background:
- Coronary artery disease (CAD) is a major cause of mortality and a complex systemic condition.
- Investigating gene expression in peripheral blood mononuclear cells (PBMC) offers insights into systemic disease processes.
Purpose of the Study:
- To determine the expression profiles of long non-coding RNAs (lncRNAs) in PBMCs from CAD patients.
- To identify potential lncRNA biomarkers for subclinical cardiac abnormalities.
Main Methods:
- lncRNA expression profiling using Illumina Hiseq 4000 sequencer on PBMCs from 40 CAD patients and 10 controls.
- Validation of differentially expressed lncRNAs via quantitative real-time polymerase chain reaction (qRT-PCR).
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for functional prediction.
Main Results:
- Significant differential expression of lncRNAs observed: 83 upregulated and 114 downregulated (P<0.05).
- Expression profiles of 48 lncRNAs correlated with CAD severity.
- GO analysis linked dysregulated lncRNAs to chromatin organization and protein heterodimerization; pathway analysis implicated viral carcinogenesis and autoimmune diseases.
Conclusions:
- lncRNAs in PBMCs represent potential biomarkers for subclinical cardiac abnormalities in CAD.
- Further research is necessary to validate these preliminary findings and their clinical utility.
Background:
Coronary artery disease (CAD) is a leading cause of death and often presents as a complex systemic disease. The aim of the presents study was to determine the expression profiles of long non-coding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMC) of CAD patients and controls.
Methods:
The lncRNA expression profiling of PBMC obtained from 40 CAD patients and 10 non-obstructive coronary atherosclerosis (NOCA) subjects were analyzed using the Illumina Hiseq 4000 sequencer. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the differentially expressed lncRNAs. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of mRNA were conducted to predict biologic functions.
Results:
Our results indicated that lncRNAs were differentially expressed; 83 were upregulated lncRNAs and 114 were downregulated lncRNAs (P<0.05). A horizontal comparison of lncRNA expression indicated that the change in the expression profile of 48 lncRNAs was consistent with the degree of CAD. Six lncRNAs were validated using qRT-PCR, confirming the accuracy of the RNA sequencing analysis. GO analysis indicated that these dysregulated lncRNA transcripts were related to chromatin organization, cell and cell part, and protein heterodimerization activity. Pathway analysis indicated that these differentially expressed genes mainly included viral carcinogenesis, systemic lupus erythematosus and alcoholism.
Conclusions:
Our preliminary findings indicate that lncRNAs could be used as potential biomarkers of subclinical cardiac abnormalities in the PBMC of CAD patients. However, further studies are needed to verify our findings and hypothesis.
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