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Assessment of progressive alterations in collagen organization in the postoperative conjunctiva by multiphoton
Li-Fong Seet1,2,3,4,5, Stephanie W L Chu1,6, Xiao Teng7,8
1Ocular Therapeutics and Drug Delivery, Singapore Eye Research Institute, Singapore.
Abstract:
Glaucoma filtration surgery (GFS) commonly fails due to excessive fibrosis. As collagen structure aberrations is implicated in adverse fibrotic progression, this study aims to uncover collagen organization alterations during postoperative scarring. Via quantitative second harmonic generation/two photon excitation multiphoton imaging, we reveal the scar development and phenotype in the mouse model of conjunctival scarring. We also show that multiphoton imaging corroborated the collagen ultrastructure anomaly characteristic of the SPARC-/- mouse postoperative conjunctiva. These data improve our understanding of postoperative conjunctival scarring and further enhance the utility of this model for the development of anti-fibrotic therapeutics for GFS.
