Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concurrent Loss of PIGA and ZRSR2 in a Patient With Paroxysmal Nocturnal Hemoglobinuria and Myelodysplastic Neoplasm.

International journal of laboratory hematology·2026
Same author

Australian and New Zealand Glaucoma Society (ANZGS) Consensus on Interventional Glaucoma.

Clinical & experimental ophthalmology·2026
Same author

ROTEM versus conventional coagulation tests in evaluating coagulopathy and transfusion requirement in ECMO patients: A retrospective study.

Perfusion·2026
Same author

Comparative Evaluation of Comprehensive DNA and RNA Sequencing Platforms with Subsequent Clinical Validation for Hematolymphoid Malignancies.

Cancers·2026
Same author

Immunophenotypic, Genetic, and Clinical Features Associated With RUNX1 Mutation in Acute Leukemias and Chronic Myeloid Neoplasms.

International journal of laboratory hematology·2026
Same author

Small areas of damage due to glaucoma are less likely to be identified by SITA-Faster than SITA-Standard.

Optometry and vision science : official publication of the American Academy of Optometry·2026

Related Experiment Video

Updated: Nov 27, 2025

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.5K

Malaria Screening Using Front-Line Loop-Mediated Isothermal Amplification.

Eric McGinnis1, Geoffrey Chan2, Monika Hudoba1,2

  • 1Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.

American Journal of Clinical Pathology
|December 7, 2020
PubMed
Summary

Loop-mediated isothermal amplification (LAMP) significantly reduced malaria testing workload and labor time in a multicenter region. This novel malaria diagnostic algorithm maintained efficiency without compromising turnaround times.

Keywords:
LAMPLoop-mediated isothermal amplificationMalaria diagnosisMalaria screening

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.9K

Related Experiment Videos

Last Updated: Nov 27, 2025

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.5K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.9K

Area of Science:

  • Clinical Diagnostics
  • Infectious Disease Research
  • Molecular Diagnostics

Background:

  • Microscopy and rapid diagnostic tests (RDTs) are standard malaria diagnostic methods.
  • Reliance on microscopy can be resource-intensive and requires skilled personnel.
  • Developing efficient and accessible malaria screening tools is crucial for public health.

Purpose of the Study:

  • To evaluate the implementation of loop-mediated isothermal amplification (LAMP) as a front-line malaria screening test.
  • To assess the impact of LAMP on test volumes, positivity rates, turnaround times, and labor time.
  • To determine the efficacy of a novel malaria testing algorithm in a multicenter hub-and-spoke model.

Main Methods:

  • A multicenter health region implemented LAMP-based malaria testing in October 2018.
  • Data from 2016-2019 were reviewed, comparing pre- and post-LAMP implementation periods.
  • LAMP was used as a front-line screening test for hub facility investigations and satellite facility investigations with negative RDTs and microscopy.

Main Results:

  • Blood film microscopy and RDT workloads decreased by 90% and 46%, respectively, post-LAMP implementation.
  • Patient numbers and malaria positivity rates remained similar compared to historical data.
  • LAMP turnaround times were comparable to RDTs, with no increase in RDT and microscopy turnaround times.

Conclusions:

  • LAMP was successfully integrated into the regional malaria diagnostic algorithm.
  • The implementation significantly reduced reliance on microscopy and RDTs.
  • Substantial labor time savings were achieved without compromising diagnostic efficiency or turnaround times.