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Updated: Nov 27, 2025

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
Published on: September 10, 2016
Optical sensors of heterotrimeric G protein signaling
Alexey Bondar1,2,3, Josef Lazar1,2
1Center for Nanobiology and Structural Biology, Institute of Microbiology of the Czech Academy of Sciences, Nove Hrady, Czech Republic.
Abstract:
Heterotrimeric G proteins are central mediators of cellular signal transduction. They receive, process, and transduce signals from G protein-coupled receptors to downstream effectors. Since their discovery, a number of optical sensors of G protein localisation and function have been developed and applied in living systems. In this minireview, we provide an overview of existing G protein-based sensors and the experimental approaches they utilise, with emphasis on live-cell imaging techniques. We outline recent advances, as well as identify current challenges and likely future directions in the field of G protein sensor development.
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